114P Identification of cholangiocarcinoma-derived exosomal proteins biomarkers

Exosomes (EXO) in cholangiocarcinoma (CCCs) are distinctly strong intermediator with ability for flipping the action of neighbouring cells. This becomes more obvious that exosomes have aptitude to encourage development pre metastatic niche. As metastasis happen, cells need migrate a new environment which also requires being favourable tumor colonize properly. Their release into circulation has potential inform about status. In-depth proteomic characterization plasma-derived EXOs been limited by challenges isolating EXO s from protein-abundant biological fluids. We implemented novel single-step density gradient flotation workflow efficient and rapid isolation highly enriched circulating exosomes. Effective methods still waiting there is high confidence identification blood-based biomarkers. recently emerged as source circulatory biomarkers cancer. NTA TEM confirmed presence (EXOs) isolated differential centrifugation. Total proteins extracted 40 Cholangiocarcinoma healthy subjects was analysed. FACS analysis characterized exosomal tetraspanins CD63, CD9 CD81 demonstrated be significantly increased patients controls. Western blot blood carried out identify showing altered levels cases comparison The result shows were detected individuals. Proteomic Our findings support derived complement other approaches cancer assessment help computational protein-protein interactions their role disease pathogenesis.